Part I: Introduction to biology (5h50 CM)

- Basis of Biology, Immunology and Microbiology

Part II: Bioanalytical chemistry (21h: 17h30 CM, 3h30 TD)

- Purification of biomolecules (liquid chromatography, gel electrophoresis)
- Characterization of proteins using MS-based approaches (bottom-up, top-down, data-dependent acquisition, data-independent acquisition)
- Development of proteomic strategies for global and targeted analyses, including protein quantification
- Structural proteomics (native MS, HDX, crosslinking)
- Case studies: analysis of publications

Part III: Contribution of miniaturization for biomolecules (11h40: 10h30 CM, 1h10 TD)

- Introduction. Interests and specificities of nanometric scale for separation methods. Transport mode, separation modes and dispersion effects.
- Last insights in nanometric scale for chromatography, from micro to nano flow rate. Evolution of new stationary phases, i.e. monoliths.
- Introduction to electrophoretic separation. From 2D Gel to capillary electrophoresis. Overview of the different detection modes (UV, Fluorescent, MS)
- Lab on chip, the future of miniaturization for separation methods.
- Example of miniaturization for health application.
 

Objectif de l’UE
Analytical sciences play an important role at the chemistry-biology interface. They help to understand complex biological systems and contribute to the development of new diagnostic and therapeutic approaches. They are, for example, essential for the increasingly detailed characterization of biomolecules such as therapeutic proteins. Analytical sciences play also a crucial role in the separation and quantification of small molecules as well macromolecules in biological samples (biological fluids, tissues, cells). This course will focus on the separation and characterization methods adapted to biomolecules and biological samples, including the development of miniaturized methods.

Compétences développées et/ou évaluées :
In the term of the lecture, the students should be able:
- to set up a purification strategy adapted to biomolecules and their complexes
- to design a convenient MS-based proteomic strategy for the identification and quantification of proteins in complex biological samples
- to understand the principles used specifically in nanoscale analytical techniques, the basics of microfluidics, nanochromatography, capillary electrophoresis
- to identify the similarities and differences between the macro and the micro scale techniques
- to practice calculations of some parameters that control the different systems used (electroosmotic flow or theoretical plates height for example)

 

Fundamentals of liquid chromatography and mass spectrometry