L’Institut de Physique et de Chimie des Matériaux de Strasbourg vous propose un séminaire intitulé « Fluorescent organic nanoparticles for biosensing » présenté par Andrey S. Klymchenko, (du Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg) le vendredi 19 juin 2026 à 15h dans l’Auditorium de l’IPCMS.
Fluorescent organic nanoparticles (NPs) emerged as an attractive platform for designing functional nanomaterials, including fluorescent biosensors. Particularly promising are dye-loaded polymer[1] and lipid[2] NPs, inspired from the field of drug delivery.
To assemble polymeric NPs of controlled size of 10-40 nm, we developed an approach of nanoprecipitation of hydrophobic polymers bearing a few charged groups.[3] To ensure dye encapsulation with minimal aggregation-caused quenching, bulky hydrophobic counterions were proposed as nano-spacers within dyes.[4] Close dye proximity with minimal aggregation ensured ultrafast dye-dye energy migration rendering NPs unprecedented light-harvesting properties[5] and enabled long-range energy transfer breaking the Forster law.[6] Their functionalization with DNA yielded nanoprobes for amplified detection of RNA/DNA markers (Fig. 1A) of cancer[7] and viral diseases.[8]
In the second approach, we developed fluorescent lipid NPs,[2] which are self-assembled from reagents generally recognized as safe. Their good stability in vivo[9] suggested them as promising nanocarrier of contrast agents and drugs as well as nanoreactor for biosensing. By combining molecular recognition with dynamic imine chemistry inside this lipid nanoreactor, we introduced a concept of artificial receptor for neurotransmitter sensing.[10] Further coupling of molecular recognition with an irreversible reaction inside the nanoreactor enabled us to reach nM sensitivity to the neurotransmitter target.[11
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- A. Reisch, D. Heimburger, P. Ernst, A. Runser, P. Didier, D. Dujardin, A. S. Klymchenko, Adv. Funct. Mater. 2018, 28, 1805157.
- A. Reisch, P. Didier, L. Richert, S. Oncul, Y. Arntz, Y. Mely, A. S. Klymchenko, Nature Commun. 2014, 5, 4089.
- K. Trofymchuk, A. Reisch, P. Didier, F. Fras, P. Gilliot, Y. Mely, A. S. Klymchenko, Nat. Photonics 2017, 11, 657.
- D. S. Biswas, P. Gaki, E. Cruz Da Silva, A. Combes, A. Reisch, P. Didier, A. S. Klymchenko, Advanced Materials 2023, 35, 2301402.
- a) N. Melnychuk, S. Egloff, A. Runser, A. Reisch, A. S. Klymchenko, Angew. Chem. Int. Ed. 2020, 59, 6811; b) S. Egloff, N. Melnychuk, A. Reisch, S. Martin, A. S. Klymchenko, Biosens. Bioelectron. 2021, 179, 113084.
- E. Cruz Da Silva, P. Gaki, F. Flieg, M. Messmer, F. Gucciardi, Y. Markovska, A. Reisch, S. Fafi-Kremer, S. Pfeffer, A. S. Klymchenko, Small 2024, e2404167.
- R. Bouchaala, L. Mercier, B. Andreiuk, Y. Mely, T. Vandamme, N. Anton, J. G. Goetz, A. S. Klymchenko, J. Control. Release 2016, 236, 57.
- B. Kozibroda, J.-M. Lehn, A. S. Klymchenko, Angewandte Chemie International Edition 2025, e202419905.
- B. Kozibroda, J.-M. Lehn, A. S. Klymchenko, Journal of the American Chemical Society 2026, 148, 21420.
